- ￥1200
- 博尔森生物
- BES2039K-1-1PL
- 中国/上海
- 2025年11月26日
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- 详细信息
- 技术资料
- 品系：
人
- 细胞类型：
正常细胞
- 肿瘤类型：
否
- 供应商：
上海博尔森生物科技有限公司
- 库存：
大量
- 英文名：
ERK1 Knockout Hela Cell Ripa Lysate (Pool)
- 生长状态：
详见操作说明
- 年限：
12个月
- 运输方式：
4℃
- 器官来源：
人
- 是否是肿瘤细胞：
否
- 细胞形态：
人
- 免疫类型：
详见操作说明
- 物种来源：
人
- 相关疾病：
详见操作说明
- 组织来源：
详见操作说明
- 规格：
50μL, 2μg/μl
ERK1基因敲除Hela细胞RIPA裂解液（Pool）
ERK1 Knockout Hela Cell Ripa Lysate (Pool)
Catalog Number BES2039K-1-1PLERK1 Knockout Hela Cell Ripa Lysate (Pool)
Gene Symbol MAPK3
Synonyms ERK; ERK-1; ERT 2; ERT2; Extracellular Signal Regulated Kinase 1; Extracellular signal related kinase 1; Extracellular signal-regulated kinase 1; HGNC6877; HS44KDAP; HUMKER1A; Insulin Stimulated MAP2 Kinase; Insulin-stimulated MAP2 kinase; MAP
kinase 1; MAP kinase 3.
gRNA Species Human
Parental Cell line Hela
Knockout Region E2
Knockout Validation PCR + Sanger Sequencing
Mutation Description Knockout achieved by using CRISPR/Cas9, Deletion of the selection cassette in exon 2.
Applications: WB
Packaging 1 vial knockout cell Lysate and 1 vial parental cell Lysate.
Shipping Conditions 4℃
Storage Store at -20°C for 6 months storage or -80°C for long-term storage.
Notes This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background The protein encoded by this gene is a member of the MAPkinase family. MAP kinases, also known as extracellularsignal-regulated kinases (ERKs), act in a signaling cascade thatregulates various cellular processes such as proliferation,differentiation, and cell
cycle progression in response to avariety of extracellular signals. This kinase is activated byupstream kinases, resulting in its translocation to the nucleuswhere it phosphorylates nuclear targets. Alternatively splicedtranscript variants encoding different protein isoforms have beendescribed.
Protocol Lysates are made using Ripa buffer with 1mM Phenylmethylsulfonyl fluoride (PMSF).
Proteins in this buffer are denatured.To use as WB control, SDS-PAGE loading buffer should be added to the lysate and boiled for 5 minutes, then loaded with gel.
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ERK1 Knockout Hela Cell Ripa Lysate (Pool)
￥1200
