2-BroMo-N-Methyl-1,3-thiazole-

DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE

immediately before using. Stocks X-Phos (100X):10mg/ml 5-bromo-4-chloro-3-indolyl-phosphate (also referred to as BCIP) in H20. Store in the dark as aliquots at -200 C. Can be frozen and thawed several times. NBT (50X):50 mg/ml nitro

Characterization of Histaminergic Receptors

N ‐α‐methyl histamine 10,000 42,000 2.6 23 d RBI R ‐α‐methyl histamine 57,000 >100,000 2.1 146 d RBI S ‐α

燕麦转基因及其在提高渗透胁迫耐受中的应用

磷酸酶缓冲液：100 mmol/L Tris-base，100 mmol/L NaCl，5 mmol/L  MgCl2( pH 9.8 ) 含有  0.33 mg/ml  NBT  (Nitro  blue  tetrazolium ) ，0.16 mg/ml BCIP (5 - bromo - 4 -chloro-3 -indolyl  phosphate)。 ( 7 ) GUS底物：10 mmol/L EDTA  ( pH 7.0 ) ，0.1 mol/L phosphate  buffer  (pH