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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8℃
- 英文名:
5-Methyl-4-nitro-2-propyl-2H-pyrazole-3-carboxylic acid
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
39658-17-8
- 规格:
1g
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文献和实验) Dissolve 0.5 grams of Eosin Y in 100 ml of water. Ethanol (C H OH MW 46.07) Density = 0.7893 gm/ml 50-95% (v/v) Since 95% ethyl alcohol is less expensive and easier
Transformation of Compenent E. coli[Yale University]
by adding 20 mM Tris pH8 to the Y medium. Michael Koelle has reproduced this success. TfbI: 30mM potassium acetate 1.5g 100mM RbCl2 6.0g 10mM CaCl2 * 2H20 0.74g 50mM MnCl2 * 4H20 4.95g Add glycerol to 15% (v/v) 75ml H2O to 500ml pH to 5.8 with ~10
at a concentration of 10 mM (1080 ul are needed for one batch each of A, C, G, and T mixes, so slightly more than five tubes will be needed-each tube contains 200 ul). 20 mM dATP 20 mM dCTP 20 mM dTTP 95 ul of 100 mM dATP 95 ul of 100 mM dCTP
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