4-nitroso-1lambda6-thiomorphol

DNA的分子量估算，别人给的。

为在72℃，30分钟内将10nmol同位素标记的4×dNTP掺入到DE81纸不溶物质中所需Pfu酶的量。四、10′PCR 反应缓冲液： 100mM KCl，160mM (NH4),2SO4，20mM MgSO4，200mM Tris·HCl，pH8.8，1% Triton X-100，1 mg/ml BSA五、质量控制： 经过检测无外源核酸酶活性；SDS-PAGE检测纯度大于95%；PCR方法检测无外源DNA；能从100ng lambda模板中扩增出1kb和2kb的单拷贝基因。六、储存条件： Pfu

AFLP: not only for fingerprinting, but for positional cloning

° C), 1.0% Triton X-100; Mg-free) 1.2 µL 25 mM MgCl2 0.08 µL (0.4 U) Taq Pol (Promega) 14.42 µL dH2O PCR reactions (recipe is per rxn) 1 µL cut/ligated nucleic acid (from above) [equals 10 ng total nucleic acid) 19 µL pre-amplification

AFLP: not only for fingerprinting, but for positional cloning

[10x = 100 mM Tris-acetate, pH 7.5, 100 mM Mg-acetate, 500 mM K-acetate) w/ 250 ng/µg BSA (=5x OPA+- BSA) Q.S. to 40 µL w/ dH20 - incubate @ 37° C for ~ 3 hrs [Note: The digestion should not be done for significantly longer than 3 hrs