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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8℃
- 英文名:
MONO-METHYL POLYETHYLENE GLYCOL 5,000 GLYCIDYL ETHER
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
40349-67-5
- 规格:
100mg
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文献和实验Crystallization of Kinesin Family Motor Proteins
temperature11 mg/ml protein 10 mM HEPES pH 7.5, 150 mM NaCl, 1 mM DTT, 1 mM MgCl2, 0.2 mM NaN3, 2 mM ADP combined 1:1 with 22% methyl ether PEG 2000, 100 mM NaCl, 2% ethylene glycol, 50 mM HEPES pH 7.0Gulick et al., 1998Ncd 281-700Sitting drop 4°C20 mg/ml
of de-fined diatomaceous earth in guanidine-HCl (100 mg/ml) to each bottle. Allow the DNA to bind at room temperature for 5 minutes with occasional mixing. Centrifuge at 9,000 for 10 minutes in the RC5-B using the GS3 rotor. 10b. Decant the supernatant
Methods for DNA isolation(DNA分离方法)
-containing supernatant is transferred to a new tube,and the plasmid or cosmid DNA is precipitated by the addition of polyethylene glycol and collected by centrifugation.The DNA pellet is resuspended in a buffer containing cesium chloride and ethidium bromide
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