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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 英文名:
PALMITOLEIC ACID ARACHIDYL ESTER
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
22522-34-5
- 规格:
100mg
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文献和实验CHROMATIN IMMUNOPRECIPITATION (CHIP) PROTOCOL FOR YEAST
centrifuge (SS34, 4℃). Cool large rotor (TMA-3E) for TOMY centrifuge. Day 1 Crosslinking Cells When culture is ready (usually I aim for OD 600 = 1.2; I"ve used up to 1.5), add formaldehyde directly to the medium
of de-fined diatomaceous earth in guanidine-HCl (100 mg/ml) to each bottle. Allow the DNA to bind at room temperature for 5 minutes with occasional mixing. Centrifuge at 9,000 for 10 minutes in the RC5-B using the GS3 rotor. 10b. Decant the supernatant
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
stock solution Nutrient-rich fly medium Phosphate-buffered saline (PBS; pH 7.5) Poly-L-lysine solution (0.1% [w/v] in H2 O; Sigma P8920) Sodium phosphate buffer (10 mM, pH 6.8) Triton X-100 VECTASTAIN Elite ABC Kit
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