L-A-GLYCEROPHOSPHORYLETHANOLAM

Bacterial transformation

  Introduction Transformation is the process of introducing foreign DNA (e.g plasmids, BAC) into a bacterium. Bacterial cells into which foreign DNA can be transformed are called competent . Some bacteria are naturally competent (e.g B

CTAB法制备超螺旋质粒DNA

中沉淀核酸的特性， 并且能基于线状、环状及超螺旋质粒 DNA 构象的差异， 使质粒与 CTAB 间的中和反应在分子间按序发生，超螺 旋质粒 DNA 处于拓扑缠绕状态，其与 CTAB 的吸附能 力较弱，最后从溶液中沉淀出来. 利用此方法还可去除 蛋白质、RNA、内毒素等杂质[9] . 本研究用 CTAB 沉淀 法从 E. coli DH5α中提取 pcDNA3 质粒…… 全文下载： http://img.dxycdn.com/trademd/upload/asset

E.coli genotypes(大肠杆菌基因型)

is not listed, it is not known to be mutated. Prophages present in wt K-12 strains (F, λ, e14, rac) are listed only if absent. E. coli B strains are naturally lon- and dcm-. F- = Does not carry the F plasmid F