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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8°C
- 英文名:
4-Nonyl Phenol-13C6 Monoethoxylate
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
1346600-52-9
- 规格:
0.5mg
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文献和实验Protein extraction from whole tissues for IEF
of methanol 80% acetone (100 ml) - store at -20 C 70% ethanol (100 ml) - store at -20 C IEF Sample Buffer (10 ml) 8 M urea 4.85 g 2 M thiourea 1.52 g 2% CHAPS 0.2 g 2% Triton X-100 200 ul 50 mM DTT 8 mg/ml make to 10 ml
of de-fined diatomaceous earth in guanidine-HCl (100 mg/ml) to each bottle. Allow the DNA to bind at room temperature for 5 minutes with occasional mixing. Centrifuge at 9,000 for 10 minutes in the RC5-B using the GS3 rotor. 10b. Decant the supernatant
30 minutes. 5. Add 300μl of CIAP stop buffer. Phenol:chloroform extract and ethanol precipitate by adding 0.5 volume 7.5M ammonium acetate (pH 5.5) and 2 volumes of 100% ethanol to the final aqueous phase. [b]Note: CIAP may be added directly
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