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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 英文名:
Bis(2-Hydroxyethyl)-3-Aminopropyltriethoxysilane
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
7538-44-5
- 规格:
5g
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文献和实验Preparation of linear polyacrylamide used as carrier in ethanol precipitation of nucleic a
Preparation of linear polyacrylamide used as carrier in ethanol precipitation of nucleic acids Linear polyacrylamide can be used as an efficient neutral carrier for precipitating nucleic acids with ethanol (Gaillard, C
Gene Transfer into Mammalian Cells Using Calcium Phosphate and DEAE-Dextran
, especially use of N,N-bis (2-hydroxyethyl)-2-aminoethanesulfonic acid (the BES variation), has proven to be the only method sufficient for the cotransfection of multiple plasmids into a wide variety of cell types. Although not as widely used today, DEAE
The ribonuclease protection assay (RPA)
, then spin in a microfuge for 2 minutes at RT. 5. Transfer the upper aqueous phase to a new 1.5 ml Eppendorf tube and add 50 µl 4 M ammonium acetate and 250 µl ice cold 100% ethanol. Invert the tube to mix and incubate for 30 minutes at -70°C. Spin
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