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- HPA040260-100UL
- 2025年11月28日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
RT
- 保质期：
见标签
- 库存：
999
- 供应商：
鹿森生物
- 规格：
100ul
说明
免疫原
sorbitol dehydrogenase recombinant protein epitope signature tag (PrEST)
应用
All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.
The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information.
特点和优势
Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.
Every Prestige Antibody is tested in the following ways:
IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
Protein array of 364 human recombinant protein fragments.
联系
Corresponding Antigen APREST81490
外形
Solution in phosphate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide.
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文献和实验Human Platelet Antigen Genotyping by PCR-SSP in Neonatal/Fetal Alloimmune Thrombocytopenia
(see Note 1 ). Maternal anti-HPA IgG antibodies can cross the placenta and cause the immune destruction of fetal platelets. Severe fetal thrombocytopenia can result in intracerebral haemorrhage (ICH) or death in utero . Unlike hemolytic disease
. 5 ml 1 M Tris-HCl, pH 8.0 2 ml 0.5 M EDTA 2 ml Triton X-100 (Sigma X-100) ddH2O to 100 ml TTE: 10 mM Tris-HCl, pH 8.0, 0.5% Triton X-100, and 0.1 mM EDTA in double distilled water. 500 ul 1 M Tris-HCl, pH 8.0 250 ul Triton X
下 12000rpm 离心 15 min。收集蛋白上清，取少许裂解液用于后续检测各蛋白表达情况。剩余的裂解液加入 Anti-Cdc37，4℃ 缓慢摇晃孵育过夜。用适量裂解缓冲液预处理 ProteinA+G，20uL/管加入各抗体孵育过夜的细胞裂解液中，4℃ 缓慢摇晃孵育 2~4 h。免疫沉淀反应后，4℃，3000rpm，3 min，小心吸取上清，琼脂糖珠用 1 mL 裂解液冲洗 3-4 次，最后加入 15uL 2*SDS 上样缓冲液，100℃，5 min。Laemmli-SDS-PAGE电泳：电泳时根据情况
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