Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-APOD Polyclonal Antibody, Unconjugated(bs-1254R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
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- bs-1254R
- 2025年10月25日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1254R |
| 英文名称 | Apolipoprotein D Rabbit pAb |
| 中文名称 | 载脂蛋白D抗体 |
| 英文别名 | Apolipoprotein D; APOD; APO D; APOD protein; APOD_HUMAN; Apolipoprotein D; Apo-D; ApoD. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Dog, Pig, Cow, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human APOD |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 19 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Tumor biomarkers Cardiovascular > Lipids / Lipoproteins > Lipoproteins/Apolipoproteins Cardiovascular > Lipids / Lipoproteins > Lipoproteins/Apolipoproteins > Apolipoproteins Metabolism > Types of disease > Neurodegenerative disease Neuroscience > Neurology process > Neurodegenerative disease > Alzheimer's disease Signal Transduction > Metabolism > Lipid metabolism |
| 亚基 | Homodimer. In plasma, also exists as a disulfide-linked heterodimer with APOA2. |
| 亚细胞定位 | Secreted. |
| 组织特异性 | Expressed in liver, intestine, pancreas, kidney, placenta, adrenal, spleen, fetal brain tissue and tears. |
| 翻译后修饰 | N-glycosylatd. N-glycan heterogeneity at Asn-65: Hex5HexNAc4 (major) and Hex6HexNAc5 (minor); at Asn-98: Hex5HexNAc4 (minor), dHex1Hex5HexNAc4 (major), dHex1Hex6HexNAc5 (minor) and dHex1Hex7HexNAc6 (minor). |
| 相似性 | Belongs to the calycin superfamily. Lipocalin family. |
| 功能 | APOD occurs in the macromolecular complex with lecithin-cholesterol acyltransferase. It is probably involved in the transport and binding of bilin. Appears to be able to transport a variety of ligands in a number of different contexts. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Apolipoprotein D (APO D), a glycoprotein involved in the human plasma lipid transport system. It is a progesterone binding glycoprotein of 24,000 dalton monomer molecular size, is a constituent of high density lipoprotein in plasma. The function of apolipoprotein D in the metabolism of plasma lipoproteins is unclear but the observation that this protein forms complexes with lecithin: cholesterol acyltransferase has led to the suggestion that apolipoprotein D may be involved in cholesterol esterification and transport of substrates and products of the reaction. Apolipoprotein D is expressed in a range of normal tissues including axillary apocrine glands, adrenal cortex and corpus luteum. Peripheral nerves, pituitary, testis, cerebellum and renal tubes are also positive. APO D was first isolated in large quantity as GCDFP 24, the major protein component of most human breast cyst fluids. In recent studies, Apo D immunoreactivity has been shown to be an early indicator of prostate cancer and advanced primary prostate tumors. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti-Apolipoprotein D (bs-1254R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19 kD
Observed band size: 30 kD
A549(Human) Cell Lysate at 30 ug
Primary: Anti-Apolipoprotein D (bs-1254R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19 kD
Observed band size: 30 kD
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文献和实验该产品被引用文献
[IF={{ 14.9 }}] {Ma Pingchuan. et al. Promotion effect of TGF-β-Zfp423-ApoD pathway on lip sensory recovery after nerve sacrifice caused by nerve collateral compensation. INT J ORAL SCI. 2023 Jun;15(1):1-14} {IF} {Rat}
相关实验
| PubMed | ChemPort | 8. Burnett, J. R. & Barrett, P. H. Apolipoprotein B metabolism: tracer kinetics, models, and metabolic studies. Crit. Rev. Clin. Lab. Sci. 39, 89–137 (2002) | PubMed | ChemPort | 9. Farese, R. V. Jr, Ruland, S. L., Flynn, L. M
Overview of the Measurement of Lipids and Lipoproteins in Mice
and triglyceride metabolism. J. Clin. Invest. 114:1493‐1503. Bouly, M., Masson, D., Gross, B., Jiang, X.C., Fievet, C., Castro, G., Tall, A.R., Fruchart, J.C., Staels, B., Lagrost, L
Models and Analysis of Atherosclerosis, Restenosis, and Aneurysm Formation in the Mouse
, X., Charnigo, R., Howatt, D.A., Rateri, D.L., and Daugherty, A. 2009. AngII infusion promotes abdominal aortic aneurysms independent of increased blood pressure in hypercholesterolemic mice. Am. J. Physiol. Heart Circ. Physiol. 296:H1660‐H1665
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Apolipoprotein D Rabbit pAb(bs-1254R)-50ul/100ul/200ul
￥1180 - 2800
