Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CXCL16 Polyclonal Antibody, Unconjugated(bs-1441R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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- bs-1441R
- 2025年10月25日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1441R |
| 英文名称 | CXCL16 Rabbit pAb |
| 中文名称 | CXC趋化因子16抗体 |
| 英文别名 | CXC-chemokine ligand 16; Chemokine(C X C motif) ligand 16; CXC chemokine ligand 16; CXCLG16; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; SCYB16; Small inducible cytokine B16 precursor; SRPSOX; Transmembrane chemokine CXCL16; CXL16_HUMAN. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=3ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from the middle of human CXCL16 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 27 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Immunology > Innate Immunity > Chemokines > Alpha Chemokines (CXC) Immunology > Innate Immunity > Macrophage / Inflamm. |
| 亚细胞定位 | Cell membrane. Secreted. Also exists as a soluble form. |
| 组织特异性 | Expressed in T-cell areas. Expressed in spleen, lymph nodes, lung, kidney, small intestine and thymus. Weak expression in heart and liver and no expression in brain and bone marrow. |
| 翻译后修饰 | Glycosylated. |
| 相似性 | Belongs to the intercrine alpha (chemokine CxC) family. |
| 功能 | Acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | CXCL16 acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis. It induces a strong chemotactic response and calcium mobilization. It binds to CXCR6/Bonzo. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {3ug/Test} |
Images kindly provided by Dr. Denny Cottle from the publication, Fetal inhibition of inflammation improves disease phenotypes in Harlequin Ichthyosis.Formalin-fixed and paraffin embedded mouse skin probed with Rabbit Anti-CXCL16 Polyclonal Antibody (bs-1441R) at 1:50 for two hours at room temperature (green channel).
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-CXCL16 antibody (bs-1441R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-CXCL16 antibody (bs-1441R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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文献和实验该产品被引用文献
[IF={{ 9.2 }}] {Jiang Wei. et al. CD8 T cells induce the peritubular capillary rarefaction during AKI to CKD transition. INT J BIOL SCI. 2024 May;20(8):2980-2993} {IF} {Mouse}
[IF={{ 8.063 }}] {Hu ZB et al. Dysbiosis of intestinal microbiota mediates tubulointerstitial injury in diabetic nephropathy via the disruption of cholesterol homeostasis. Theranostics. 2020 Feb 3;10(6):2803-2816.} {IHC,WB,IF} {rat}
[IF={{ 6.68 }}] {Cottle, Denny L., et al. "Fetal inhibition of inflammation improves disease phenotypes in harlequin ichthyosis." Human molecular genetics (2014): ddu459.} {Other} {="Mouse"}
[IF={{ 6.291 }}] {Changjiang Liu. et al. JNK and Jag1/Notch2 co-regulate CXCL16 to facilitate cypermethrin-induced kidney damage. ECOTOX ENVIRON SAFE. 2022 Jun;238:113582} {WB,IF} {Rat}
[IF={{ 4.9 }}] {Matthew D. Lauver. et al. The CXCR6-CXCL16 axis mediates T cell control of polyomavirus infection in the kidney. PLOS PATHOG. 2025 Mar;21(3):e1012969} {IF,IHC,WB} {Mouse}
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CXCL16 Rabbit pAb(bs-1441R)-50ul/100ul/200ul
￥1180 - 2800
