Sample:
_x000D_
Small intestine (Mouse) Lysate at 40 ug
_x000D_
Primary: Anti- eIF3F (bs-3841R) at 1/1000 dilution
_x000D_
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_
Predicted band size: 38 kD
_x000D_
Observed band size: 38 kD
_x000D_
- ￥1180 - 2800
- Bioss
- bs-3841R
- 2026年10月17日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-3841R |
| 英文名称 | eIF3F Rabbit pAb |
| 中文名称 | 真核翻译起始因子3F抗体 |
| 英文别名 | eIF 3 epsilon; eIF3 p47; eIF3 p47 subunit; EIF3S5; Eukaryotic translation initiation factor 3 subunit 5; Eukaryotic translation initiation factor 3 subunit F; translation initiation factor 3 47 kda subunit; EIF3F_HUMAN. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Mouse (Human, Rat, Chicken, Dog, Cow, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human eIF3F |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 38 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > DNA / RNA > Translation > Regulation Epigenetics and Nuclear Signaling > RNAi > Eukaryotic Initiation factors (eIF's) |
| 亚基 | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with RNF139; the interaction leads to protein translation inhibitions in a ubiquitination-dependent manner. Interacts with DTX1, the interaction is required for deubiquitinating activity towards NOTCH1. |
| 亚细胞定位 | Cytoplasm. |
| 翻译后修饰 | Phosphorylated. Phosphorylation is enhanced upon serum stimulation. |
| 相似性 | Belongs to the eIF-3 subunit F family. Contains 1 MPN (JAB/Mov34) domain._x000D_ |
| 功能 | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling._x000D_ |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | EIF3F, (also known as eukaryotic translation initiation factor 3 subunit 5, EIF-3 epsilon, and EIF3 p47 subunit), is part of the EIF3 complex, which is composed of at least 12 subunits. It binds the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA. It can bind the COP9 signalosome and the 26S proteasome, possibly having regulatory functions in both protein translation and degradation. EIF3F also associates with the complex p170-EIF3. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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eIF3f Rabbit pAb(bs-3841R)-50ul/100ul/200ul
￥1180 - 2800
