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- bs-13530R
- 2025年10月25日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-13530R |
| 英文名称 | GPR31 Rabbit pAb |
| 中文名称 | G蛋白偶联受体31抗体 |
| 英文别名 | GPR31_HUMAN; 12-(S)-hydroxy-5, 8, 10, 14-eicosatetraenoic acid receptor; 12-(S)-HETE receptor; 12-HETER; G-protein coupled receptor 31; G protein-coupled receptor 31; GPR31/12-HETER; HETER; HETER1; |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human G protein coupled receptor 31 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 35 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Signaling Pathway > G Protein Signaling > GPCR |
| 亚细胞定位 | Cell membrane; Multi pass membrane protein. |
| 相似性 | Belongs to the G-protein coupled receptor 1 family. |
| 功能 | GPCR GPR31 is a member of the G protein coupled receptor (GPCR) family. Members of this protein family contain seven putative transmembrane domains and may mediate signaling processes to the interior of the cell via activation of heterotrimeric G proteins. GPCR GPR31 is located on human chromosome 6q27. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Enables G protein-coupled receptor activity and arachidonic acid binding activity. Involved in G protein-coupled receptor signaling pathway and response to acidic pH. Located in plasma membrane. [provided by Alliance of Genome Resources, Apr 2022] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Primary: Anti-GPR31 (bs-13530R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 36 kD
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文献和实验[IF={{ 2.9 }}] {Mohamed Moustafa. et al. 12-HETE activates Müller glial cells: the potential role of GPR31 and miR-29. PROSTAG OTH LIPID M. 2023 Dec;:106805} {IF} {Mouse}
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
.g., Sigma) 50 mg/ml bis‐sulfosuccinimdylsuberate (BS3 ;Pierce, cat. no. PI 21580) in dimethylsulfoxide (DMSO) 5 mg/ml
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