3,5-DIBROMO-4-HYDROXYBENZYL AL

Crystallization of Kinesin Family Motor Proteins

) PEG 4000, 2.5 mM ATP, 10 mM MgCl2Reservoir: 100 mM Na-acetate pH 4.6, 150 mM KCl, 7% PEG 4000, 5 mM ATP, 20 mM MgCl2Kull et al., 1996Ncd 335-700Sitting drop Room temperature7 mg/ml protein in 10 mM Pipes, pH 7.2, 100 mM NaCl, 1 mM EGTA, 1 mM DTT, 7% (w

C. Elegans 基因组DNA的制备

. If necessary, heat to 65C to dissolve. Adjust final volume to 100 ml.3) Protease K; 20 mg/ml in TE pH 8.0. Store at -20℃ in single use aliquots. DO NOT FREEZE-THAW.Worm preparation1) Seed 4 x 10 cm plates with 15 young adult hermaphrodites and grow 1 week

Hints and precautions for the care, feeding and breeding of Neurospora

or amino nitrogen inhibits crossing. Keep amino acid, purine or pyrimidine supplements at a minimal level in crossing medium. If total amino acids do not exceed 0.3 mg/ml there is usually no problem with fertility. Usually the parent with the simplest