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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8℃
- 英文名:
2-aMino-2-(3,4-difluorophenyl)acetonitrile
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
218449-30-0
- 规格:
100mg
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文献和实验Amino-allyl Reverse Transcription
with the amino-allyl dye coupling procedure all Tris must be removed from the reaction to prevent the monofunctional NHS-ester Cye dyes from coupling to free amine groups in solution. Fill one Microcon 30 concentrator with 450 ul water. Add neutralized
Revised amino-allyl labeling protocol
) 8 m L 100 mM aminoallyl-dUTP** (Sigma, #A0410) 2 m L 100 mM dTTP **Dissolve 10 mg aminoallyl-dUTP in 170 m L water. Add approx. 6.8 m L 1N NaOH. Final pH is roughly
Protocol for Reverse Transcription and Amino-allyl Coupling of RNA
dATP, dGTP, dCTP (Pharmacia) 8 mL 100 mM aminoallyl-dUTP** (Sigma, #A0410) 2 mL 100 mM dTTP **Dissolve 10 mg aminoallyl-dUTP in 170 mL water. Add approx. 6.8 mL 1N NaOH. Final pH is roughly 7.0 using pH paper. ***Altering
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