- ￥350
- 源叶
- 中国
- T28349-1ml
- 2025年10月29日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-80℃（运输条件：冰袋低温运输）
- 英文名：
6-Carboxyfluorescein N-hydroxysuccinimide ester
- 库存：
99
- 供应商：
上海源叶生物科技有限公司
- CAS号：
92557-81-8
- 规格：
1ml
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文献和实验, 2min at 2,000rpm. 8. Transfer to 5ml wash buffer for 20min. 9. Dry briefly and resuspend in 1ml T.E. (can be left overnight) 10. Add 1µl 10mg/ml RNA se to each 1ml T.E./DNA mixture and incubate for 60min at 37 ℃. (If RNase in the sample
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
411400 (Boehringer). For 10mM stock, weigh out 1mg (light sensitive!), add 27ul DMSO to solubilize, then add 12ul 3M NaOAc, pH5, mix, then 275ul H2O, Don''t make up large amountrs as this is not very stable, even at -80C. Diluted luciferin solution
8. Transfer to 5ml wash buffer for 20min. 9. Dry briefly and resuspend in 1ml T.E. (can be left overnight) 10. Add 1µl 10mg/ml RNA se to each 1ml T.E./DNA mixture and incubate for 60min at 37 ℃. (If RNase in the sample doesn't matter -stages 11
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