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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 英文名:
6-chloro-3-hydroxy-pyridine-2-carbonitrile
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
727736-70-1
- 规格:
100mg
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文献和实验96-well RNA In Situ Hybridization Protocol
Volume 10X dig NTP mix 25.0ul 10mM digoxigenin UTP 4.5ul 100mM UTP 7.0ul 100mM CTP 7.0ul 100mM GTP 7.0ul 100mM ATP 19.5ul ddH2 O 70.0ul Total Volume 10X DNase I Buffer 0.2M Tris pH 8 0.1M MgCl2 DNase I mix
96-well RNA In Situ Hybridization Protocol
25.0μl10mM digoxigenin UTP 4.5μl100mM UTP 7.0μl100mM CTP 7.0μl100mM GTP 7.0μl100mM ATP 19.5μlddH2 O 70.0μlTotal Volume 10X DNase I Buffer 0.2M Tris pH 8 0.1M MgCl2 DNase I mix 700μlddH2 O 100μl0.1M DTT 100μl10X DNaseI Buffer 100μ
Methods for use with the mTn-3xHA/lacZ-mutagenized library
are increased. Cells are resuspended in 1 ml of One Step buffer containing 1 mg of denatured salmon sperm DNA. 100 ul aliquots of this suspension are then added to tubes containing from 0.1 to 1 ug of Not I digested plasmid DNA. Tubes
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