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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
避光,RT,充氩
- 英文名:
Phenylbutanammonium Chloride
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
30684-06-1
- 规格:
50mg
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文献和实验Standard hybridization technique
The standard solution typically used for both pre-hybridisation and hybridisation is based on that given in Maniatis et al.,(1982)with both Denhart's solution and heterologous DNA being replaced by heparin (Singh and Jones,1984
purification. Run DNA on a 0.7% Agarose gel (use High quality agarose and TAE buffer). Before cutting out bands, rinse gel with dH2O to get rid of any adaptors that may be in gel buffer. Gerl purify using the protocol from Qiagen's QIAquick Spin Handbook
Standard hybridization technique
on that given in Maniatis et al.,(1982)with both Denhart's solution and heterologous DNA being replaced by heparin (Singh and Jones,1984). You will need: 6 x SSC (0.9M NaCl,90mM sodium citrate,pH 7) 10% SDS 50mg/ml heparin
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