- ￥7000
- 博尔森生物
- BES2088K-1-3P
- 中国/上海
- 2025年10月24日
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- 详细信息
- 文献和实验
- 技术资料
- 品系：
人
- 细胞类型：
正常细胞
- 肿瘤类型：
否
- 供应商：
上海博尔森生物科技有限公司
- 库存：
大量
- 英文名：
Calreticulin Knockout Hela Cell Line (Pool)
- 生长状态：
详见操作说明
- 年限：
12个月
- 运输方式：
Dry Ice
- 器官来源：
人
- 是否是肿瘤细胞：
否
- 细胞形态：
人
- 免疫类型：
详见操作说明
- 物种来源：
人
- 相关疾病：
详见操作说明
- 组织来源：
详见操作说明
- 规格：
1-5×10^6/vial
Calreticulin Knockout Hela Cell Line (Pool)
Gene Symbol CALR
Synonyms Autoantigen RO; CALR; CALR protein; CALR_HUMAN; cC1qR; CRP55; CRTC; Endoplasmic reticulum resident protein 60; ERp60; grp60; HACBP; RO; Sicca
syndrome antigen A; SSA.
gRNA Species Human
Parental Cell line Hela
Knockout Region E2
Knockout Validation PCR + Sanger Sequencing
Mutation Description Knockout achieved by using CRISPR/Cas9, Deletion of the selection cassette in exon 2.
Number of Cells 1-5×10⁶
Packaging 1 vial knockout cell line and 1 vial parental cell line.
Shipping Conditions Dry Ice
Storage Stored in liquid nitrogen for a long time.
Notes This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Calreticulin is a highly conserved chaperone protein which resides primarily in the endoplasmic reticulum, and is involved in a variety of cellular processes, among them, cell adhesion. Additionally, it functions in protein folding quality control and calcium homeostasis. Calreticulin is also found in the nucleus, suggesting that it may have a role in transcription regulation. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin. Recurrent mutations in calreticulin have been linked to various neoplasms, including the myeloproliferative type.
Protocol Upon arrival，please resuscitate the cells as soon as possible for culture to confirm cell vitality, status and preservation.
1.Thaw the vial in 37℃ water bath，and gently shake it to accelerate its dissolution.
2.Transfer the cell suspension to a 15mL aseptic centrifuge tube with pre-warmed 5mL complete medium，and centrifuge 1000rpm for approximately 5 minutes at room temperature.
3.Discard supernatant after centrifugation.
4.Resuspend the cell pellet with 1mL pre-warmed complete medium, and transferred to a T75 culture flask.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
5.Add 15-20mL complete medium.
6.Incubate the culture at 37℃ incubator with 5% CO₂.
7.Cultures should be monitored daily.
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文献和实验指从某初代培养开始通过连续培养所产生的所有细胞。包括从初代培养的细胞和细胞群所发展的一系列的细胞。如果培养的状态清楚，则在前面冠以有限继代性（ finite）或连续继代性（ continuous）。例如，人二倍体成纤细胞系属于前者， HeLa细胞系属后者。另外连续继代性细胞系也称为树立细胞系。
Gene Knockout Techniques(1) The Knockout Process
). Spin cell debris to bottom of wells (2000 RPM/2 minutes). Pool by sampling 75 ul from each well of a plate with the Hydra and dispensing 70 ul into a sterile 8-channel pooling block (this looks like a tiny little swimming pool with 8 horizontal lanes
的突变的结果。对产生危机早的小鼠和仓鼠等确立细胞株比较容易做到，而人胎儿成纤维细胞株的确立是困难的。在进行确立细胞株时，细胞容易发生恶性性状转变，从纯系动物体细胞建立的确立细胞株，对于来源动物（作为细胞株来源的动物）可引起肿瘤的形成。确立细胞株最早是由 W． R． Earle（ 1943）从 C3H系小鼠分离出的 L细胞株，其次是 G． O． Gey（ 1952）由人子宫癌分离出的海拉（ HeLa）细胞。确立细胞株一般说容易培养，增殖能力强，可进行定量处理，所以能在试管内繁殖，用作多细胞生物体的细胞
