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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
避光;-20°C;避免反复冻融
- 英文名:
alpha Tubulin Mouse mAb (Biotin)
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- 规格:
10μl
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文献和实验In situ hybridisation to alpha satellite sequences (chromosome specific)
ng/m l) 5 m l Carrier DNA Sheared salmon sperm DNA (10mg/ml) 0.5 m l H2 O _ 2 m l 20 x SSC _ 2.5 m l TOTAL
Dual- and Triple-Color Fluorospot
in the analysis, e.g., IL-10, rat mAb 9D7 is used for coating and tag (different from FITC and biotin)-labeled rat mAb 12G8 is used for detection. All mAbs from Mabtech, Nacka Strand, Sweden. 4. Secondary reagents for amplification: Mouse anti-FITC mAb
-stop PCR only; see Steps 1-22)SSCP loading dye (for SSCP only; see Steps 23-37)95% (v/v) formamide10 mM NaOH0.25% (w/v) bromophenol blue0.25% (w/v) xylene cyanolTaq DNA polymerase (5 U/µl)TBE Buffer (1X and 5X)Prepare a 5x stock solution in 1 liter
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