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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8°C;避光;禁止冷冻
- 英文名:
Mouse IgG (Pacific Blue)
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- 规格:
25μg
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文献和实验Ab concentrations.OD (Absorbance at 280 nm)/1.35 = X mg/ml.Store Abs at -20℃ or further concentrate by using Centriprep. And store at -20℃.Regenerate Ab column with Regenerate buffer (~200 ml).
buffer (pH9.0) ~ 200 ml. Filter protein mixture Add protein mixture or culture supernatant containing Ab (adjust pH to 7.8 with Binding buffer ; red color) to the Protein A column. Mouse antibodies of the IgG1 subclass
Culturing Mouse Embryonic Fibroblasts
Save CNS or liver tissue in a 1.5 mL tube for DNA extraction and genotyping. Embryos should be signed a mouse ID and genotyped according to standard protocols. It may be necessary to dilute the DNA or limit the PCR cycle number
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