- ￥159.90
- 源叶
- 中国
- K31597-10μg
- 2025年10月27日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
2-8°C;避光;禁止冷冻
- 英文名：
Mouse IgG2a-kappa (APC), anti-A29L
- 库存：
99
- 供应商：
上海源叶生物科技有限公司
- 规格：
10μg
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文献和实验Phycoerythrin conjugation protocol
30 µl DTT (0.5M, i.e. 77 mg/ml in PBS). Incubate for 30 mins at room temperature. Separate derivatized PE and Ab from activators on G-10 columns. You should now have approximately 2 ml of each reactant. Mix derivatized
, dry 5. block half of the plate (with platelets) by PBS-BSA 1% and coat another half by glycine (x1) - BSA for 1 hour at 37ѓC 6. wash 3 times in PBS-tween 20, dry 7. incubated at 37ѓc for 1 hour with a 1:1000 dilution of rat IgG2a anti-mouse kappa
by the Bradford method. 5. Mix 50 μg of proteins with 30 μL of 2× SDS-gel samplebuffer and then boil for 5 min. 3.2. Sds-page 1. These instructions assume the use of a Hoeffer SE-600 gelsystem. 2. Prepare a 1.5-mm thick, 10% gel by mixing 8.0 mL of 4×
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