- ￥139.90
- 源叶
- 中国
- K31304-10μg
- 2025年10月27日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
2-8°C;避光;禁止冻结
- 英文名：
Recombinant CD4 Antibody (Biotin)
- 库存：
99
- 供应商：
上海源叶生物科技有限公司
- 规格：
10μg
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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
Protease inhibitor cocktail for ChIP (1000X) Proteinase K (20 mg/mL) (Roche Diagnostics) Reagents for agarose gel electrophoresis RIPA buffer for ChIP RNase A SDS (sodium dodecyl sulfate) (10%) T cell isolation buffer
Dual- and Triple-Color Fluorospot
. Spontaneous production is determined by incubating the same number of cells in the absence of antigen. A polyclonal activator, such as anti-CD3 mAb (100 ng/ml) or phytohemagglutinin (PHA; 1–10 μg/ml), should normally be used as a control for cell viability
Cell Surface Immunofluorescence Staining Protocol
-CD4-Biotin, and anti-CD8-APC) and incubate on ice for 15-20 minutes in the dark. 9) Wash 2X with at least 2 ml of Cell Staining Buffer by centrifugation at 350 x g for 5 minutes. 1) If using a purified primary antibody, resuspend
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