- ￥199.90
- 源叶
- 中国
- K31281-10μg
- 2025年10月27日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
2-8°C;避光;禁止冻结
- 英文名：
Recombinant CD3 Antibody (AF647)
- 库存：
99
- 供应商：
上海源叶生物科技有限公司
- 规格：
10μg
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文献和实验Immune Response Biomarker Profiling
Forceps and deionized water 实验步骤 Preparing buffers 1) Prepare 100 mL of the blocking buffer by mixing 5 mL of 1 M HEPES, pH 7.5, 4 mL of 5 M NaCl, 800 l of 10% Triton® X-100, 50 mL of 50% Glycerol, 610 mg
Combined Flow Cytometric Measurement of Two Cell-Surface Antigens and DNA-RNA Content
Actinomycin D (AD) stock solution (1 mg/mL) 7-aminoactinomycin D (7-AAD) stock solution (1 mg/mL) Antibody staining solution Human cells of interest, fresh or cultured (or immortalized human cell lines) Monoclonal antibodies (mAb
Intracellular Cytokine Staining Protocol
)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble) 5hrMonensinJES6-5H4IL-4mouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble) 5hrMonensinBVD6-24G2IL-6mouse
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