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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8°C;避光;禁止冻结
- 英文名:
Recombinant CX3CR1 Antibody (Biotin)
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- 规格:
10μg
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文献和实验Studies of the Ubiquitin Proteasome System
6.6 ) Basic Protocol 17: Radiolabeling Ubiquitin with 32P Materials 20 mg/ml bovine ubiquitin (Sigma‐Aldrich) in coupling buffer Coupling buffer: 50 mM sodium borate, pH 8.5 10 mg/ml Sulfo‐NHS‐LC‐Biotin (Pierce
Tissue forceps Scalpel with #22 blade 10‐, 5‐, and 1‐ml pipets
BioID: A Screen for Protein‐Protein Interactions
expressing cells. (4 ) Induce biotinylation with excess biotin prior to (5 ) cell lysis and protein denaturation. (6 ) Perform biotin‐affinity capture of biotinylated proteins. (7 ) Reserve 10% of the sample for streptavidin immunoblot analysis. Use
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