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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8°C;避光;禁止冻结
- 英文名:
Human IgG2-Kappa (FITC)
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- 规格:
10μg
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文献和实验Phycoerythrin conjugation protocol
P-801, R-PE in 60% NH4 SO4 at 4 mg/ml. 0.25 ml of P-801. Spin 10 mins on low in microfuge. Discard supernatant. Resuspend PE in 0.25 ml PBS. Dialyze twice for 30 mins versus 150 ml PBS at room temperature. Adjust PE to approx 1.4 mg
Dual- and Triple-Color Fluorospot
labeled with green fluorophore emitting light at the same wavelength as FITC, streptavidin (SA) labeled with Cy3, and if three cytokines are analyzed, a mouse anti-tag mAb labeled with Cy5 (Mabtech). 5. Mouse mAb anti-human CD28 at 0.1 mg/ml
Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional Profiling
in organ culture dishes PBS with Ca++ and Mg++ (PBS+ ) Dispase solution (10 mg/ml in hESC medium; see recipe ), ice cold
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