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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C;避免反复冻融
- 英文名:
GNA12 Antibody
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- 规格:
10μl
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文献和实验monoclonal antibody production fusion
, add ddH2 O up to 1 L Gluta x50, 1 L, pH 6.0 = glutamine, 10.8 g (1.48 mM) + HCl, 0.5N, 100 ml -> heat to 37ѓC, add ddH2 O up to 1 L aminopterine x100, 0.22-0.45 µm-filtered, store at -20ѓC = aminopterine, 4.4 mg (3.8µM)+ ddH2 O
ELISA Procedure for Measuring Serum Antibody Titer
. 2. Remove the unbound conjugate by washing with PBS-T. 3. Add 200 µL of a 10 mg/mL so, lution of p-nitrophenyl phosphate in 0.05 M sodium carbonate buffer (pH 9.8, containing 1 mM MgCl2), slowly at room temperature until a yellow color develops
Antibody Staining of Dissected Gonads
at least 4 hr at RT or overnight at 4 oC (Overnight at 4 oC is probably best). *Wash in PBTw, 3-4 x 5 min *Wash in PBS containing 100 ng/ml DAPI. *Wash in PBTw, once *Place in 80% glycerol containing anti-fade reagent (e.g., Dapco) about 20 30 ul
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