Recombinant Human Calnexin/CAN

美国实验室WESTERN BLOT 方法

WESTERN BLOT PROTOCOL In a Western blot, proteins that are separated on polyacrylamide gels on the basis of size are transferred to a membrane for detection with antibodies. Preparation of Human Cell

免疫沉淀-蛋白印迹法

& Medicine, King College London, UK).Rainbow recombinant protein molecular protein markers, ECL western blotting detection reagents were purchased from Amersham Pharmacia Boutech (Little Chalfont, Buckinghamshire, UK).Protogel 30%acrylamide solution, 10

美国实验室wetern方法

. (In the case of the recombinant 52 kD Ro/SSA, the antigenic pellet may be stored at -20°C for about 1 month or -70°C for about 3 months.) SDS-PAGE Separating Gels (Hoeffer baby gels): 1. For 2 gels, mix in an Erlenmeyer flask in the fume hood