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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C;避免反复冻融
- 英文名:
Recombinant Human PSAP Protein
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- 规格:
10μg
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文献和实验-8878.3. Takayama, H. et al. 2008. High-level expression, single-step immunoaffinity purification and characterization of human tetraspanin membrane protein CD81. PLoS ONE 3: e2314 (DOI: 10.1371/journal.pone.0002314).4. Zhong, M. and Molday, R.S. 2010
by the Bradford method. 5. Mix 50 μg of proteins with 30 μL of 2× SDS-gel samplebuffer and then boil for 5 min. 3.2. Sds-page 1. These instructions assume the use of a Hoeffer SE-600 gelsystem. 2. Prepare a 1.5-mm thick, 10% gel by mixing 8.0 mL of 4×
v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
with the Wnt preparation and cellular context. Key words: sFRP , Recombinant expression , Protein purification , Heparin chromatography , Chelating chromatography , Wnt 1. Introduction Secreted Frizzled-related proteins (sFRPs) contain a Frizzled(Fzd
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