Recombinant Streptavidin Prote

Size and Shape of Protein Molecules at the Nanometer Level Determined by Sedimentation, Gel Filtration, and Electron Microscopy

recombinant protein. Red and purple show Hsk3p. The proteins were identified and quantitated by Western blot of the fractions, shown in c . The four protein bands eluted together at 1/D  = 0.35 × 107 , corresponding to R s  = 7.6 nm, and at 7.4 S. Reproduced

Combined Flow Cytometric Measurement of Two Cell-Surface Antigens and DNA-RNA Content

dual-laser flow cytometer with blue (488-nm) and red (633- or 647-nm) excitation. DNA is stained at low pH in the presence of saponin with 7-aminoactinomycin D (7-AAD), and RNA is stained with pyronin Y (PY). Both dyes are used at low concentration

Fluorochrome Absorption and Emission Spectra

are useful in experiments where multi-color analysis is desired using flow cytometers with dual laser capabilities (Fig 1). APC can be excited by a helium-neon (HeNe) laser emitting light at 633 nm, by a krypton laser emitting light at 647 nm, or by a dye