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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C,避免反复冻融
- 英文名:
Recombinant CLIC4 Antibody
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- 规格:
10μl
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文献和实验Dissolve in buffer A the purified antibody carrying an engineered phosphorylation site (e.g., the sequence DDDSDDDD; at 1 mg/ml concentration (alternatively, exchange buffer by dialysis). To 100 µl antibody solution, add 500 units casein
Differentiating Neural Stem Cells into Neurons and Glial Cells
Medium Antibiotic-Antimycotic solution Fetal Bovine Serum, ES Cell-Qualified FBS GlutaMAX™-I FGF-basic (AA 10–155), Recombinant Human (bFGF) EGF, Recombinant Human CELLstart™ CTS™ Geltrex™ Reduced Growth Factor
minutes at 4°C. Discard the supernatant. 3. Dissolve the pellet in 1 ml of lysate buffer. Bring to 1 mM PMSF by adding 100 mM PMSF stock (17.42 mg/ml in methanol). This solution may be kept for 1 week at -20°C. 4. Add 50 µl of a 10 mg/ml lysozyme
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