- ￥99 - 999
- Sigma
- 进口
- SML1666-1ML
- 2025年10月24日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
RT
- 保质期：
见标签
- 库存：
999
- 供应商：
鹿森生物
- 规格：
1ML
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文献和实验in methanol at room temp. 3. Remove methanol and add 1x Blotting buffer until ready to use. G. Membrane transfer 1. Assemble "sandwich" for Bio-Rad's Transblot. 2. Prewet the sponges, filter papers (slightly bigger than gel) in 1x Blotting buffer
Filter Binding Assay for EBNA-1
, 3ml syringePre-incubate filter with 100ul Binding Buffer at filter binding temperature (4C) until ready to bind DNA/EBNA-1 solution (e.g. conduct in 7th floor cold room).Incubate DNA fragment of interest with DNA with no known binding sites in a total
Isolation of Protein from Tissue
with protease inhibitors added.Add about 1ml Brij/tissues that equals about 100µl in volume.Brij with inhibitors on ice before use. Brij 150 (Lysis buffer)Tris 1M 1ml EDTA 0.5M 0.4ml NaCl5M 3ml Brij 96 10% 8.75ml NP40 10% 1.25ml QS to 100ml with H2
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