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- Sigma
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- SML1508-25MG
- 2025年11月04日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
RT
- 保质期：
见标签
- 库存：
999
- 供应商：
鹿森生物
- 规格：
25MG
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文献和实验Preparation of Segmented and Polarity Marked Microtubules
consisting of 15 µM tubulin (1 part rhodamine tubulin to 10 parts unlabeled tubulin ) and 12 µM NEM GTP-tubulin in 1X BRB80, 1 mM DTT, 1 mM GTP. Incubate at 0¡C for 5' and (optionally) clarify at 90K for 5' at 2¡C in a TLA100 rotor. 3. Incubate dim polar
Preparation of Segmented and Polarity Marked Microtubules
1. Polymerize bright GMPCPP seed mix at 37¡C for 15'-30'. 2. On ice prepare the dim polar elongation mix consisting of 15 µM tubulin (1 part rhodamine tubulin to 10 parts unlabeled tubulin ) and 12 µM NEM GTP-tubulin in 1X BRB80
CGH of Biotin Labeled DNA vs Digoxigenin Labeled DNA
cement. Let rubber cement dry 5 mins on warmer. Incubate for 2-3 days at 37 C in a humid chamber. DAY 2: WASHES AND STAINING Remove rubber cement. Slide coverslips off gently. Wash 3X for 12 mins each at 45 C in 50
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