- ￥7000
- 博尔森生物
- BES2016K-1-2P
- 中国/上海
- 2025年10月24日
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- 详细信息
- 文献和实验
- 技术资料
- 品系：
人
- 细胞类型：
正常细胞
- 肿瘤类型：
否
- 供应商：
上海博尔森生物科技有限公司
- 库存：
大量
- 英文名：
IL7 receptor alpha Knockout Hela Cell Line (Pool)
- 生长状态：
详见操作说明
- 年限：
12个月
- 运输方式：
Dry Ice
- 器官来源：
人
- 是否是肿瘤细胞：
否
- 细胞形态：
人
- 免疫类型：
详见操作说明
- 物种来源：
人
- 相关疾病：
详见操作说明
- 组织来源：
详见操作说明
- 规格：
1-5×10^6/vial
IL7 receptor alpha Knockout Hela Cell Line (Pool)
Gene Symbol IL7R
Synonyms IL-7Rα; IL-7Ra; Interleukin-7 receptor subunit alpha; CD 127; CD127; CD127 antigen; CDW127; IL 7R alpha.
Swiss P16871
Gene ID 3575
gRNA Species Human
Parental Cell line Hela
Knockout Region E5
Knockout Validation PCR + Sanger Sequencing
Mutation Description Knockout achieved by using CRISPR/Cas9, Deletion of the selection cassette in exon 5.
Number of Cells 1-5×10⁶
Packaging 1 vial knockout cell line and 1 vial parental cell line.
Shipping Conditions Dry Ice
Storage Stored in liquid nitrogen for a long time.
Notes This product as supplied is intended for research use only, not for use in human,
therapeutic or diagnostic applications.
Background The protein encoded by this gene is a receptor for interleukine 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in the V(D)J recombination during lymphocyte development. This protein is also found to control the accessibility of the TCR gamma locus by STAT5 and histone acetylation. Knockout studies in mice suggested that blocking apoptosis is an essential function of this protein during differentiation and activation of T lymphocytes. The functional defects in this protein may be associated with the pathogenesis of the severe combined immunodeficiency (SCID).
Protocol Upon arrival，please resuscitate the cells as soon as possible for culture to confirm cell vitality, status and preservation.
1.Thaw the vial in 37℃ water bath，and gently shake it to accelerate its dissolution.
2.Transfer the cell suspension to a 15mL aseptic centrifuge tube with pre-warmed 5mL complete medium，and centrifuge 1000rpm for approximately 5 minutes at room temperature. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
3.Discard supernatant after centrifugation.
4.Resuspend the cell pellet with 1mL pre-warmed complete medium, and transferred to a T75 culture flask.
5.Add 15-20mL complete medium.
6.Incubate the culture at 37℃ incubator with 5% CO₂.
7.Cultures should be monitored daily.
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文献和实验GABAA Receptor 1 Subunit (Gabra1) Knockout Mice: Review and New Results
, we present phenotypes observed in a GABAA receptor alpha-1 subunit (Gabra1) knockout line (KO). Our results are reviewed along with those from earlier publications on different Gabra1 mutant lines. We discovered a heightened anxiety profile in KO mice
Gene Knockout Techniques(1) The Knockout Process
). Spin cell debris to bottom of wells (2000 RPM/2 minutes). Pool by sampling 75 ul from each well of a plate with the Hydra and dispensing 70 ul into a sterile 8-channel pooling block (this looks like a tiny little swimming pool with 8 horizontal lanes
Analysis of B-Cell Signaling Using DT40 B-Cell Line
A chicken DT40 B-cell line provides an excellent model system for the analysis of the function of genes related to B-cell antigen receptor (BCR) signaling. Crosslinking of BCR by �-chain-specific antibody stimulates DT40 cells to undergo
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