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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 保质期:
见标签
- 库存:
999
- 供应商:
鹿森生物
- 规格:
250MG

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文献和实验filter sterililize; no DEPC treatment. Preparation of dNTP's. The quick protocol for ~100mM stock. dilute all four dNTP's (250mg of each) in 3.676ml of H2 O; + 424µl 5M NaOH; check the pH: ~0.5µl on pH-paper
Large Scale Plasmid Preps: Qiagen/Cesium Method
according to the Qiagen Maxi or Mega prep protocol. Resuspend the DNA in 3.7 mL of T.E. Add 3.7 gm. CsCl and 0.37 mL of 1 mg/mL ethidium bromide. Check the density of the DNA solusion by placing 0.01 mL (= V) on an analytical balance and recording
-ethyl-9-phenyl- phenanthridinium bromide, 250mg bottle. Fetal calf serum: HYCLONE,INC. cat# A-1111-D. Defined fetal bovine serum, 100ml bottle, store frozen. Hank's Balanced Salt Solution: GIBCO cat# 14170-112. Hank's balanced salt solution
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