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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 保质期:
见标签
- 库存:
999
- 供应商:
鹿森生物
- 规格:
250MG

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文献和实验PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)
for calculation of the Tm isTm = 4(G + C) + 2(A + T)℃.Thus, the annealing temperature chosen for a PCR depends directly on length and composition of the primer(s)。 One should aim at using an annealing temperature (Ta) about 5℃ below the lowest Tm of ther pair
, this method for preparing competent E. coli from Inoue et al. (1990) can challenge the efficiencies achieved by Hanahan (1983). However, under standard laboratory conditions, efficiencies of 1 x 108 to 3 x 108 transformed colonies/mg of plasmid DNA
) (store at 4deg.C). IPTG (isopropyl b-D-thiogalactopyranoside):25 mg/ml IPTG in double distilled water 250 mg IPTG (Sigma I-5502) ddH2 O to 10 ml (aliquot and store at -20℃) 1 M isocitrate (sodium salt-dihydrate) : 29.41 g Na3isocitrate-2H
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