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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 保质期:
见标签
- 库存:
999
- 供应商:
鹿森生物
- 规格:
100MG

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文献和实验Protocol for Trichloroacetic Acid (TCA) Precipitation
of radiolabeled nucleotide incorporation in a polymerization reaction by TCA precipitation. In the protocol, nucleic acid synthesis reactions are carried out in the presence of a radiolabeled nucleotide (e.g., 32 P-dATP or 32 P-UTP). Then, the synthesized polymers
at room temperature. Allow the cells to rehydrate for 15 minutes. 2) Counterstaining Protocol A. Dilute the DAPI stock solution to 3 μM in staining buffer (100 mM Tris, pH 7.4, 150 mM NaCl, 1 mM CaCl2 , 0.5 mM MgCl2 , 0.1% Nonidet P-40
Acid Phosphatase for Glycol Methacrylate Sections Procedure
Sodium Nitrite - 1 gm Distilled Water - 25 ml Prepare fresh and store at 4°C. Methyl Green Methyl Green (C.I.# 42585) - 1 g Phosphate/citrate buffer 0.1M pH 4.0 - 100 ml Citation: Gerrits, P. O
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