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RT
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鹿森生物
- 规格:
10G

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文献和实验Molecular Modeling of Nucleic Acid Structure: Electrostatics and Solvation
Kang, Y.K., Gibson, K.D., Nemethy, G., and Scheraga, H.A. 1988. Free energies of hydration of solute molecules. 4. Revised treatment of the hydration shell model. J. Chem. Phys. 92:4739‐4742
A quick RNA mini-prep for Neurospora mycelial cultures
temperature. [E buffer: 50 mM Tris-Cl pH 8.0, 300 mM NaCl, 5 mM EDTA, pH 8.0, 2 SDS; autoclave and add 1 mM ATA and 14 mM ß-mercaptoethanol. ATA=aurintricarboxylic acid, ammonium salt (Sigma #A0885, St. Louis, MO)] 5. Thaw the powder in E buffer in 42°C
Preparation of Competent Cells (from PMB)
#A 1X 200 ml 1X 10 mM MOPS pH 7.0 210 mg Free acid 230 mg Na salt 10 mM Rubidium chloride 240 mg Autoclave and store at 4 ℃ PMB #B 1X 200 ml 1X 10 mM MOPS pH 6.5 350 mg Free acid 85 mg Na salt 50 mM Calcium chloride 1.12 g 10 mM
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