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文献和实验Replication timing by density transfer
easier to dilute a solution that's too dense than to make a too-dilute solution more dense!) CsCl solution : Use 1.28 g dry CsCl per gram of 10 mM Tris, 100 mM EDTA , pH 7.5. The T10 E100 should have a refractive index of 1.3395
【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
., Asmann,Y. W., Thibodeau, S. N., Tessarollo, L., Copeland,N. G., Jenkins, N. A., Cormier, R. T., and Largaespada, D. A. (2009) A transposon-based genetic screen in mice identifies genes altered incolorectal cancer. Science 323, 1747–50. 14. Jenkins, N
The ribonuclease protection assay (RPA)
, then spin in a microfuge for 2 minutes at RT. 5. Transfer the upper aqueous phase to a new 1.5 ml Eppendorf tube and add 50 µl 4 M ammonium acetate and 250 µl ice cold 100% ethanol. Invert the tube to mix and incubate for 30 minutes at -70°C. Spin
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