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文献和实验Replication timing by comparative hybridization
V): Strains : R-KT-DIR and R-Zeo-DIR are both derived from RM14-3a (MAT a, cdc7 , his6 , leu2 , trp1 , ura3 , bar1 ). The ARS-less cassette in R-KT-DIR is a Kanr -TRP1 fragment integrated near the right end of chromosome V. The t
Cys C UGC Cys C UGA Stop UGG Trp W C CUU Leu L CUC Leu L CUA Leu L CUG Leu L CCU Pro P CCC Pro P CCA Pro P CCG Pro P CAU His H CAC His H CAA Gln Q CAG Gln Q CGU Arg R CGC Arg R CGA Arg R CGG Arg R A AUU Ile I AUC Ile I AUA Ile I AUG Met M ACU Thr T ACC
Two-hybrid analysis of genetic
combinations of strains,plasmids,and reporters can be used for mating (Section 3).In one common version (Finley and Brent,1994),the strain expressing the bait (bait strain)is RFY206 (MATa ura3-52 his3Æ200 leu2-3 lys2Æ201 trp1::hisG)transformed with the HIS3
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