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- 2025年10月24日
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文献和实验Lipid Component Quantitation by Thin Layer Chromatography
This chapter describes a thin layer chromatography (TLC) method for the quantitation of various lipids (such as phospholipids, sphingolipids, acylglycerols, and fatty acids) in lipid-based nanoparticle formulations such as liposomes
The Split-Ubiquitin System for the Analysis of Three-Component Interactions
Protein–protein interactions are vital to all living cells. Many different in vivo and in vitro approaches are published which enable detection and determination of interactions between two proteins. However, most of these techniques
Creation and Characterization of Component Libraries for Synthetic Biology
and the circular polymerase extension cloning (CPEC) method. Additionally the workflow of screening the raw library for functional parts is included to provide a full overview of the process of creating and characterizing a component library for synthetic biology.
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