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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 保质期:
见标签
- 库存:
999
- 供应商:
联硕生物
- 规格:
250MG

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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
immediately before using. Stocks X-Phos (100X):10mg/ml 5-bromo-4-chloro-3-indolyl-phosphate (also referred to as BCIP) in H20. Store in the dark as aliquots at -200 C. Can be frozen and thawed several times. NBT (50X):50 mg/ml nitro
Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
(Boehringer Mannheim Cat# 242 608)- Levamisole (Sigma, Cat# L-9756) - NaN3 (Sigma Cat# S-2002)- NBT: 4-Nitro blue tetrazolium chloride. (Boehringer Mannheim Cat# 1383 213)- p-Phenylenediamine (Sigma Cat# P-6001)- 0.1% Poly-L-lysine solution
AND SOLUTIONS) can indicate DNA concentration because its heaviest (20 kb band) approximates to 250 ng/µl of DNA concentration and the next two bands are 125 ng/µl and 67.5 ng/µl respectively. 7) The gel was typically run (at 100v) for 30 to 60 min
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