- ￥99 - 999
- Sigma
- 进口
- S373079-50MG
- 2025年10月24日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
RT
- 保质期：
见标签
- 库存：
999
- 供应商：
联硕生物
- 规格：
50MG
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文献和实验Preparing a Selenomethionyl Protein
, phenylalanine to the culture. Add 50mg leucine, isoleucine, valine to the culture (all as solid powders). Add 120mg DL-Se-Met or 60mg L-Se-Met to the culture (as a solid powder). Continue to grow the culture for 15 minutes. Induce with 1mL
Replication timing by density transfer
C]- acetate or glucose 0.01% (w/v) [15 N]- ammonium sulfate Amino acids/supplements (as required, added to the same concentration as in normal synthetic medium) --dissolved in "-N" medium and filtered
Preparing a Selenomethionyl Protein
and grow until an OD600 =0.5-0.6. (~2.0 - 2.5 hours) Add 100mg threonine, lysine hydrochloride, phenylalanine to the culture. Add 50mg leucine, isoleucine, valine to the culture (all as solid powders). Add 120mg DL-Se-Met or 60mg L-Se-Met
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