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文献和实验)-cellulose consists of a polymer of 10–20 T-residues, covalently linked to a cellulose matrix, which will hybridize to and bind poly(A)+ containing RNA providing the tail is at least 15–20 bases long. Ribosomal and transfer RNA do not bind efficiently
Poly(ADP-ribose) (pADPr) is a posttranslational modification that regulates protein function through two major mechanisms: covalent modification of acceptor proteins and noncovalent binding of proteins to pADPr. pADPr is synthesized
The Purification of Poly(A)-Containing RNA by Affinity Chromatography
and simple affinity chromatography procedure (1 ). Under high salt conditions (0.3–0.5M NaCl or KCl), poly(A) will hybridize to oligo(dT)-cellulose or poly(U)-Sepharose. These commercially available materials consist of polymers of about 10–20 nucleotides
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