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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 保质期:
见标签
- 库存:
999
- 供应商:
联硕生物
- 规格:
100MG

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文献和实验Installation of Site‐Specific Methylation into Histones Using Methyl Lysine Analogs
2: Alkylation Reactions to Produce Methyl Lysine Analog Histones Materials 10 mg histone pellet (from protocol
Preparing a Selenomethionyl Protein
antibiotics (1000x conc.) 20mL amino acid 50x Stock (If precipitate is seen, heat to 60-70°C and shake.) Inoculate M9 with 50mL overnight culture and grow until an OD600 =0.5-0.6. (~2.0 - 2.5 hours) Add 100mg threonine, lysine hydrochloride
Crystallization of Kinesin Family Motor Proteins
temperature11 mg/ml protein 10 mM HEPES pH 7.5, 150 mM NaCl, 1 mM DTT, 1 mM MgCl2, 0.2 mM NaN3, 2 mM ADP combined 1:1 with 22% methyl ether PEG 2000, 100 mM NaCl, 2% ethylene glycol, 50 mM HEPES pH 7.0Gulick et al., 1998Ncd 281-700Sitting drop 4°C20 mg/ml
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