3,3′-Methylene-bis(4-hydroxyco

Use of a Novel 5′‐Regioselective Phosphitylating Reagent for One‐Pot Synthesis of Nucleoside 5′‐Triphosphates from Unprotected Nucleosides

, and Klenow fragment exo(−) (Kf−). Primer was 5′‐end‐labeled using [γ‐32 P]ATP and polynucleotide kinase. Polymerization reactions were carried out with primer (3.5 µM), template (5 µM), all dNTPs and dNTPαS (0.1 mM each, final concentration), and Kf− (0.015 µ