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- 文献和实验
- 技术资料
- 保存条件:
RT
- 保质期:
见标签
- 库存:
999
- 供应商:
联硕生物
- 规格:
100MG

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文献和实验Procedures for In Vitro Production of Bovine Embryos
hood 100 ml pipet tips and pipettor OMM + supplements Mineral oil 60 x 15 petri dishes Plastic pasteur pipet 3-4 bottles of OCM + supplement (warmed @ 38.5°C) 1 L saline + Pen/Strep (warmed @ 38.5°C)Preparation for Oocyte Collection Scalpel Petri dishes
Sucrose Density Gradient Fractionation
below).Resuspend in 10 ml SK with 1 mg zymolyase and 28.8 mM Beta-mercaptoethanol to make SK:thaw zymolyase (kept at 5 mg/ml stock in SK at -20℃)at room temp,then spin 1 min at 14,000 rpm.Take supe.-- for 4 gradients,use 900μl of supe,90μl of beta-mercaptoethanol
MS, JinO, Woodgett JR. (2000) Requirement for glycogensynthase kinase-3beta in cell survival andNF-kappaB activation. Nature. 406 , 86–90. 12. Meijer L, Flajolet M, Greengard P. (2004)Pharmacological inhibitors of glycogen synthasekinase 3. Trends
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