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PKM2 Mouse pAb(bs-0102M)-50ul/

100ul/200ul
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  • ￥1180 - 2800
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  • bs-0102M
  • 2025年10月24日
  • 产品信息以Bioss网站为准
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北京博奥森生物技术有限公司
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    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-0102M
    英文名称PKM2 Mouse pAb
    中文名称丙‌‌酮酸激酶-M2抗体
    英文别名PK 1; PK 2; PK 3; PK Muscle type; PK1; PK2; Pk3; PKL; PKLR; PKM 2; PKM; PYKM; Pyruvate kinase 1; Pyruvate kinase 2/3; Pyruvate kinase 3; Pyruvate kinase isozyme R/L; Pyruvate kinase isozymes M1/M2; Pyruvate kinase liver and blood cell; Pyruvate kinase liver and RBC; Pyruvate kinase liver and RBC type; Pyruvate kinase M2; Pyruvate kinase muscle; Pyruvate kinase muscle isozyme; Pyruvate kinase type L; R type/L type pyruvate kinase; Red cell/liver pyruvate kinase; RPK; TCB; THBP 1; THBP1; Thyroid hormone binding protein cytosolic; CTHBP; Cytosolic thyroid hormone binding protein; MGC3932; OIP 3; Oip3; Tumor M2-PK; p58; OIP-3; KPYM_HUMAN.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat, U-87MG (Pig, Cow, Horse, Rabbit)
    抗体来源Mouse
    免疫原KLH conjugated synthetic peptide derived from human PKM2
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量58 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cancer > Cancer Metabolism > Metabolic signaling pathway > Metabolism of carbohydrates

    Cancer > Tumor biomarkers > Enzymes

    Epigenetics and Nuclear Signaling > Cell cycle > Kinases/Phosphatases

    Metabolism > Pathways and Processes > Metabolic signaling pathways > Carbohydrate metabolism

    Metabolism > Pathways and Processes > Metabolic signaling pathways > Energy transfer pathways > Energy Metabolism

    Signal Transduction > Metabolism > Energy Metabolism

    亚基Monomer and homotetramer. Exists as a monomer in the absence of FBP, and reversibly associates to form a homotetramer in the presence of FBP. The monomeric form binds T3. Tetramer formation induces pyruvate kinase activity. The tetrameric form has high affinity for the substrate and is associated within the glycolytic enzyme complex. Exists in a nearly inactive dimeric form in tumor cells and the dimeric form has less affinity for the substrate. Binding to certain oncoproteins such as HPV-16 E7 oncoprotein can trigger dimerization. FBP stimulates the formation of tetramers from dimers. Interacts with HERC1, POU5F1 and PML. Interacts (isoform M2) with EGLN3; the interaction hydroxylates PKM under hypoxia and enhances binding to HIF1A. Interacts (isoform M2) with HIF1A; the interaction is enhanced by binding of EGLN3, promoting enhanced transcription activity under hypoxia.
    亚细胞定位Cytoplasm. Nucleus. Note=Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity.
    组织特异性Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
    翻译后修饰ISGylated.
    Under hypoxia, hydroxylated by EGLN3.
    Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy.
    相似性Belongs to the pyruvate kinase family.
    功能Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料The protein encoded by this gene is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {ICC/IF}{1:100-500}
    {IF}{1:100-500}

     

    产品细节图片1
    Sample:
    MOLT-4(Human) Cell Lysate at 30 ug
    Primary: Anti-PKM2 (bs-0102M) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
    Predicted band size: 58 kD
    Observed band size: 58 kD
    产品细节图片2
    Sample:
    Hela Cell (Human) Lysate at 40 ug
    NIH/3T3 Cell (Mouse) Lysate at 40 ug
    Muscle (Mouse) Lysate at 40 ug
    Jurkat Cell (Human) Lysate at 40 ug
    Primary: Anti-PKM2 (bs-0102M) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
    Predicted band size: 58 kD
    Observed band size: 58 kD
    产品细节图片3
    Tissue/cell: rat colitis tissue;4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-M2-PK Polyclonal Antibody, Unconjugated(bs-0102M) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Mouse IgG, Cy5 conjugated(bs-0296G-Cy5)used at 1:200 dilution for 40 minutes at 37°C.
    产品细节图片4
    Tissue/cell: Human nasopharyngeal carcinoma; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-M2-PK Polyclonal Antibody, Unconjugated(bs-0102M) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0024) and DAB(C-0010) staining
    产品细节图片5
    Sample:
    Lane 1: A549 (Human) Cell Lysate at 30 ug
    Lane 2: U87MG (Human) Cell Lysate at 30 ug
    Lane 3: U251 (Human) Cell Lysate at 30 ug
    Primary: Anti-PKM2 (bs-0102M) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
    Predicted band size: 60 kD
    Observed band size: 60 kD
    产品细节图片6
    Hela cell; 4% P‌‌araformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (PKM2) polyclonal Antibody, Unconjugated (bs-0102M) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
    产品细节图片7
    U-87MG cell; 4% P‌‌araformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (PKM2) polyclonal Antibody, Unconjugated (bs-0102M) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

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    图标文献和实验
    该产品被引用文献

    [IF={{ 7.4 }}] {Xue Li. et al. Pantothenate Kinase 4 Governs Lens Epithelial Fibrosis by Negatively Regulating Pyruvate Kinase M2-Related Glycolysis. AGING DIS. 2023 Oct 1; 14(5): 1834–1852} {IF} {Mouse,Human}

    [IF={{ 3.9 }}] {Xu Wei-long. et al. Quercetin Antagonizes Glucose Fluctuation Induced Renal Injury by Inhibiting Aerobic Glycolysis via HIF-1α/miR-210/ISCU/FeS Pathway. Front Med-Lausanne. 2021 Mar;8:219} {WB} {Mouse}

    [IF={{ 3.1 }}] {Huang Hui. et al. Osthole inhibits GSK-3β/AMPK/mTOR pathway-controlled glycolysis and increases radiosensitivity of subcutaneous transplanted hepatocellular carcinoma in nude mice. STRAHLENTHER ONKOL. 2023 Nov;:1-9} {WB} {Mouse,Human}

    [IF={{ 12.4 }}] {Jingyi Wang. et al.A novel pathway for stemness propagation and chemoresistance in non-small cell lung cancer via phosphorylated PKM2-loaded small extracellular vesicles..Theranostics.2025 Feb 24;15(8):3439-3461.} {IHC} {Human,Mouse}

    [IF={{ 1.1 }}] {Xiang Yijia. et al. Scutellarin Protects against Myocardial Ischemia–reperfusion Injury by Enhancing Aerobic Glycolysis through miR-34c-5p/ALDOA Axis. INT J APPL BASIC MED. 2024 May;14(2):85} {WB} {Rat}

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    文献支持
    PKM2 Mouse pAb(bs-0102M)-50ul/100ul/200ul
    ￥1180 - 2800