Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-DFFB Polyclonal Antibody, Unconjugated(bs-0043R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
- ￥1180 - 2800
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- bs-0043R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0043R |
| 英文名称 | DFFB Rabbit pAb |
| 中文名称 | Caspase 激活的脱氧核糖核酸酶抗体 |
| 英文别名 | CAD; 5730477D02Rik; Caldesmon; Caspase activated deoxyribonuclease; Caspase activated DNase; Caspase activated nuclease; Caspase-activated deoxyribonuclease; Caspase-activated DNase; Caspase-activated nuclease; CDM; CPAN; Deoxyribonuclease III, caspase activated; DFF 40; DFF-40; DFF2; DFF40; DFFB_HUMAN; Didff; DNA fragmentation factor 40 kDa subunit; DNA fragmentation factor subunit beta; DNA fragmentation factor, 40 Da, beta polypeptide(caspase activated DNase); DNA fragmentation factor, 40 kD beta subunit; DNA fragmentation factor, 40 kD, beta polypeptide; DNA fragmentation factor, 40kDa, beta polypeptide(caspase activated DNase); DNA fragmentation factor, 40kDa, beta polypeptide(caspase-activated DNase); DNAation factor, beta subunit; OTTHUMP00000003633. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Rabbit, GuineaPig) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from mouse DFF-40 beta |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 40 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cell Death > Apoptosis > Metabolism Cancer > Cell Death > Apoptosis > Nucleus > DNAses Cell Biology > Apoptosis > Nucleus > Chromatin Condensation Cell Biology > Apoptosis > Nucleus > DNAses Epigenetics and Nuclear Signaling > Cell cycle > Apoptosis > Nuclear Metabolism > Pathways and Processes > Metabolism processes > Apoptosis |
| 亚基 | Heterodimer of DFFA and DFFB. Interacts with HIST1H1A. |
| 亚细胞定位 | Cytoplasm. Nucleus. |
| 相似性 | Contains 1 CIDE-N domain. |
| 功能 | Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of these variants has not been determined. [provided by RefSeq, Jul 2008]. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DFFB) Polyclonal Antibody, Unconjugated (bs-0043R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DFFB) Polyclonal Antibody, Unconjugated (bs-0043R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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文献和实验该产品被引用文献
[IF={{ 5.076 }}] {Endesfelder Stefanie. et al. Prevention of Oxygen-Induced Inflammatory Lung Injury by Caffeine in Neonatal Rats. Oxid Med Cell Longev. 2020;2020:3840124} {IHC} {Rat}
[IF={{ 3 }}] {Andreas Pietrucha. et al. Oxygen and HIF1α-dependent SDF1 expression in primary astrocytes. DEV NEUROBIOL. 2024 Mar;:} {WB} {Mouse}
[IF={{ 1.647 }}] {Yuan J et al. Bmp4 inhibits goose granulosa cell apoptosis via PI3K/AKT/Caspase-9 signaling pathway. (2018) Anim Reprod Sci. 200:86-95.} {WB} {Goose}
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DFFB Rabbit pAb(bs-0043R)-50ul/100ul/200ul
￥1180 - 2800
